Protective effect of hyperbaric oxygen therapy on gentamicin-induced nephrotoxicity


ÖZTOPUZ R. Ö. , TÜRKÖN H. , ŞEHİTOĞLU H. , BÜYÜK B. , UZUN M. , OVALI M. A. , ...More

7th International Molecular Biology and Biotechnology Congress, Konya, Turkey, 25 - 27 April 2018, pp.48

  • Publication Type: Conference Paper / Summary Text
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.48

Abstract

Hyperbaric O2 is a method of using pure O2 at a higher atmospheric pressure in order to
treat a medical condition. Hyperbaric oxygen therapy (HBO) for gentamicin-induced
nephrotoxicity is thought to be effective in the treatment of renal toxicity in animal models.
The purpose of this study is to investigate the effect of HBO therapy on gentamicin-
induced nephrotoxicity in rats. Rats were randomly assigned to four different groups
of seven rats in each group. The study consists totally 28 male wistar albino rats. Fourteen
of the rats were injected with 100 mg/kg intraperitoneal gentamicin once daily for 7
days. The other half of the rats were exposed to HPO 90 min daily for 7 days under 2.5
atm. On the day of eight, the laboratory results were obtained from serum. Moreover, we
also performed malondialdehyde, Superoxide dismutase and α–Glutathione-S-Transferases
levels of the kidney in all groups. When the gene expression of cytokines in kidney
tissue was examined, TNF-α, IL-1β and Kim-1 levels in the gentamicin treatment group
were statistically increased compared to the HBO+Gentamicin group (p=0.015, p=0.024,
p=0.004). Serum Urea, albumin and LDH levels were found to be increased (p = 0.006, p
= 0.224 and p = 0.180 respectively) in gentamicin group compared to HPO + gentamicin
group. The HPO + Gentamicin therapy group was not statistically different from the control
groups but significantly different from the Gentamicin group for antioxidant parameters.
Histopathologic studies have been performed in which hyperbaric oxygen administration
significantly reduced the renal damage. Gentamicin administration caused
tubular necrosis in kidney. HBO administration may be recommended for treatment
of nephrotoxicity originating from gentamicin. Therefore, in this study, we
investigated the effects of HPO to discuss the potential role of HPO in nephrotoxicity.
Keywords: Gentamicin, HPO treatment, nephrotoxicity