Unraveling a Novel Strain of Endophytic Bacillus spp. as a Key Player in Nitenpyram Biodegradation and Brassica rapa Stress Tolerance
Journal of Soil Science and Plant Nutrition, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s42729-026-03601-1
- Dergi Adı: Journal of Soil Science and Plant Nutrition
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Natural Science Collection (ProQuest), Biological Science Database (ProQuest)
- Anahtar Kelimeler: Biodegradation, Nitenpyram neonicotinoid, Strain DN-1, Stress alleviation
- Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet
Özet
Nitenpyram is a neonicotinoid insecticide widely used to protect crops from pests. The indiscriminate use of this insecticide exerts significant negative pressure on the environment, thereby underscoring the need for sustainable removal. In the present study, we isolated a novel endophytic bacterium, Bacillus cereus strain DN-1, from Brassica rapa var. perviridis plant samples which can degrade nitenpyram in soil, liquid media, and within plant tissues. In liquid media, 90% degradation of the applied nitenpyram was observed by strain DN-1 after 28 d of incubation, and significant relative bacterial density of strain DN-1 was recorded in the time course graph. A nitroreduction pathway was identified based on the detection of degradation product, 2-[N-(6-chloro-3-pyridylmethyl)-N-ethyl]amino-2-methyliminoacetic acid (CPMF). Synergistic interactions were indicated by microcosm studies, which demonstrated enhanced biodegradation in both sterile and non-sterile soils inoculated with strain DN-1, with faster degradation under natural microbial conditions. Moreover, the nitenpyram residue concentration was significantly lower in plant tissues inoculated with strain DN-1. Further studies revealed that the application of the degrading strain to plants under nitenpyram stress improved physiological parameters, including relative water content, chlorophyll levels, and antioxidant responses, while reducing oxidative damage markers, underscoring its role in stress alleviation. This study highlights the potential of the endophytic B. cereus strain DN-1 for effective bioremediation of nitenpyram residues, enhancement of crop resilience, and promotion of sustainable agricultural practices.