Hypobaric-hypoxia-induced pulmonary damage in rats ameliorated by antioxidant erdosteine


Uzun O., Balbay O., ÇOMUNOĞLU N., Yavuz O., Annakkaya A. N. , Gueler S., ...Daha Fazla

ACTA HISTOCHEMICA, cilt.108, ss.59-68, 2006 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 108 Konu: 1
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1016/j.acthis.2006.01.001
  • Dergi Adı: ACTA HISTOCHEMICA
  • Sayfa Sayıları: ss.59-68

Özet

Free radical-mediated injury to lung and pulmonary vasculature is an important mechanism in hypoxia-induced lung damage. In this study, we aimed to investigate the potential protective effects of erdosteine as an antioxidant agent on hypobaric hypoxia-induced pulmonary hypertension. Adult mate rats were assigned randomly to three groups. The first group of rats was exposed to hypobaric-hypoxia and the second group was treated with erdosteine (20 mg/kg, daily) for 2 weeks, during which time they were in a hypoxic chamber. These groups were compared with normoxic controls. All. rats were sacrificed after 2 weeks. The hypoxia-induced increase in right ventricle to left ventricle plus septum weight ratio (from 0.20+/-0.01 to 0.26+/-0.01) was reduced significantly in the erdosteine-treated group (0.23+/-0.01). Malondialdehyde levels were elevated (from 0.33+/-0.11 to 0.59+/-0.02) and total antioxidant status was not changed significantly (from 1.77+/-0.42 to 2.61+/-0.23) by hypoxia. In contrast to the hypoxia-exposed group, malondialdehyde levels were significantly decreased in the erdosteine-treated group (0.37+/-0.02). Total antioxidant status (4.03+/-0.22) was significantly higher in erdosteine-treated rats when compared to non-treated rats. Histopathotogical examination demonstrated that erdosteine prevented inflammation and protected lung parenchyma and pulmonary endothelium of hypoxia-exposed rats. (C) 2006 Elsevier GmbH. All rights reserved.

Free radical-mediated injury to lung and pulmonary vasculature is an important mechanism in hypoxia-induced lung damage. In this study, we aimed to investigate the potential protective effects of erdosteine as an antioxidant agent on hypobaric hypoxia-induced pulmonary hypertension. Adult male rats were assigned randomly to three groups. The first group of rats was exposed to hypobaric–hypoxia and the second group was treated with erdosteine (20 mg/kg, daily) for 2 weeks, during which time they were in a hypoxic chamber. These groups were compared with normoxic controls. All rats were sacrificed after 2 weeks. The hypoxia-induced increase in right ventricle to left ventricle plus septum weight ratio (from 0.20±0.01 to 0.26±0.01) was reduced significantly in the erdosteine-treated group (0.23±0.01). Malondialdehyde levels were elevated (from 0.33±0.11 to 0.59±0.02) and total antioxidant status was not changed significantly (from 1.77±0.42 to 2.61±0.23) by hypoxia. In contrast to the hypoxia-exposed group, malondialdehyde levels were significantly decreased in the erdosteine-treated group (0.37±0.02). Total antioxidant status (4.03±0.22) was significantly higher in erdosteine-treated rats when compared to non-treated rats. Histopathological examination demonstrated that erdosteine prevented inflammation and protected lung parenchyma and pulmonary endothelium of hypoxia-exposed rats.