THE EFFECT OF SAMBUCUS NIGRA EXTRACT ON GALLERIA MELLONELLA TOTAL HEMOCYTE COUNT


Taşkın D., Öztürk R., Kaya S.

III. International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 1 - 03 September 2021, pp.211

  • Publication Type: Conference Paper / Summary Text
  • City: Edirne
  • Country: Turkey
  • Page Numbers: pp.211
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

European elder (Sambucus nigra) is distributed in Europe, Asia, and North Africa. It is used in

traditional treatments as diaphoretic, diuretic, astringent, emollient, and emetic. At the same

time, health support products containing S. nigra are offered for sale in pharmacies. Many

studies have shown that the fruits of S. nigra are antioxidant and anticarcinogenic. Its effects

on cardiovascular diseases and its anti-inflammatory properties have been demonstrated in

clinical trials. The greater wax moth (Galleria mellonella) is a preferred model organism in

immunity studies because its innate immunity characteristics have similarities with mammals.

This invertebrate model organism is preferred for research with human pathogens because it

survives at 37ºC and the data obtained from G. mellonella are comparable to mammalian

models. This study was carried out to determine the effect of S. nigra on the total hemocyte

count of the model organism G. mellonella. Dried S. nigra fruits taken from herbalists were

extracted with 70% ethanol in a soxhlet device. The stock solution was prepared by dissolving

the extract in distilled water (DW) at a rate of 500 mg mL-1. 500 to 5 mg mL-1 dilutions of this

stock solution were determined as experimental doses. According to the results of our study, it

was determined that S. nigra increased the hemocyte count significantly compared to the control

and DW groups at doses of 25 mg mL-1 and above. Whether this increase in hemocyte count

is due to a mutagenic effect or to stimulating immune responses should be clearly demonstrated.

According to these data, the effects of S. nigra fruit extract, which causes an increase in

hemocyte count at all doses, on immunity should be examined in more detail.