The objective of this research was to determine the effects of different tractor power take-off (PTO) options on working characteristics of single disc fertilizer spreader. The experiments were carried out for three PTO options, three gears and disc fertilizer spreader's three orifice position. In the study, the fertilizer spread patterns and evaluation parameters (fuel consumption, work width, effective field capacity and application rate) were determined. The experiments were conducted according to factorial experimental designs with 3 replications and examined effects of factors and their interactions on measurement parameters. In conclusion, the 540E PTO option provided the definite advantages in terms of fuel consumptions compared to the other PTO options. However, it was determined that 750 PTO option has some advantages on machine's work width and effective field capacity. In all applications trapezoidal pattern was produced by spinner spreader. Applications with 750 PTO option produced more uniform spread patterns than the other applications. Consequently, important knowledge for researchers and farmers using disc fertilizer spreaders were reported by considering the all experimental data.