Akademik Veri Yönetim Sistemi
12th International Medicine and Health Sciences Researches Congress, Ankara, Türkiye, 18 - 19 Mart 2023, cilt.1, sa.1, ss.576-585
Abstract: Since shoes are in constant contact with the external environment, they are open to microbial
contamination. Thus, it can cause the spread of both pathogenic and non-pathogenic microorganisms. Therefore,
in our study, the microbial load in the shoes of 16 young individuals aged 18-25 was determined, and the effect of
various factors on the microbial load was evaluated. Samples were taken from the inner surface of 16 pairs of
shoes by swab, and the samples were cultivated on Plate Count Agar (PCA) for total aerobic mesophilic bacteria
count and on Saboroud Dextrose Agar (SCAF) media containing Chloramphenicol and Dichloran Rose Bengal
Chloramphenicol Agar (DRBC) for mould yeast count. As a result, it was determined that the bacterial load was
>1000 CFU in 5 participants, while bacterial load could not be detected in one participant. In ten participants, it
was determined that the bacterial load ranged between 12.50 ± 5.50 and 248.50 ± 19.50 CFU. It was determined
that the mould load ranged between 3.50 ± 0.50 and 86.00 ± 28.00 CFU in the DRBC medium and between 2.50
± 0.50 and 65.50 ± 18.50 CFU in SCAF medium. It was determined that the factors affecting the microbial load
did not create a statistical difference in bacterial and fungal load. Considering the colony morphology from DRBC
and SCAF medium, 76 of 87 isolates were identified at the genus level. Acremonium, Aspergillus, Alternaria,
Cladosporium, Conidiobolus, Fusarium, Lomentospora, Penicillium, Sporotrichum and Trichophyton genera
were determined on the inner surface of the shoes.