Ayakkabı İçi Mikrobiyal Yük ve Küf Çeşitliliğinin Belirlenmesi


Özcan Ateş G., Uyanık S., Uyanık M.

12th International Medicine and Health Sciences Researches Congress, Ankara, Turkey, 18 - 19 March 2023, vol.1, no.1, pp.576-585

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.576-585
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

Abstract: Since shoes are in constant contact with the external environment, they are open to microbial contamination. Thus, it can cause the spread of both pathogenic and non-pathogenic microorganisms. Therefore, in our study, the microbial load in the shoes of 16 young individuals aged 18-25 was determined, and the effect of various factors on the microbial load was evaluated. Samples were taken from the inner surface of 16 pairs of shoes by swab, and the samples were cultivated on Plate Count Agar (PCA) for total aerobic mesophilic bacteria count and on Saboroud Dextrose Agar (SCAF) media containing Chloramphenicol and Dichloran Rose Bengal Chloramphenicol Agar (DRBC) for mould yeast count. As a result, it was determined that the bacterial load was >1000 CFU in 5 participants, while bacterial load could not be detected in one participant. In ten participants, it was determined that the bacterial load ranged between 12.50 ± 5.50 and 248.50 ± 19.50 CFU. It was determined that the mould load ranged between 3.50 ± 0.50 and 86.00 ± 28.00 CFU in the DRBC medium and between 2.50 ± 0.50 and 65.50 ± 18.50 CFU in SCAF medium. It was determined that the factors affecting the microbial load did not create a statistical difference in bacterial and fungal load. Considering the colony morphology from DRBC and SCAF medium, 76 of 87 isolates were identified at the genus level. Acremonium, Aspergillus, Alternaria, Cladosporium, Conidiobolus, Fusarium, Lomentospora, Penicillium, Sporotrichum and Trichophyton genera were determined on the inner surface of the shoes.