Methanol extracts obtained from endemic Stachys sivasica Kit Tan & Yildiz, Stachys anamurensis Sumbul, Stachys cydni Kotschy ex Gemici & Leblebici, Salvia aytachii Vural & Adiguzel, and Verbascum gypsicola Vural & Aydogdu have been investigated for their antimicrobial activity. Antimicrobial activity was determined with Escherichia coli ATCC 11230, Stapylococcus aureus ATCC 6538P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis CCM 2067, Listeria monocytogenes ATCC 15313, Micrococcus luteus CCM 169, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403, and Kluyveromyces fragilis ATCC 8608 by the disk-diffusion method. Verbascum gypsicola extracts had strong antimicrobial activity against the gram-positive bacteria and the yeast cultures. The extracts of Stachys L. were effective only against bacteria. The extracts of Salvia aytachii demonstrated an antimicrobial effect against bacteria and the yeast cultures used in this study.