Paper discusses the results of the evaluations done on the basis of the data obtained from the 3-years field study on the effects of different irrigation regimes and water stress imposed at different growth stages on plant growth of flue-cured tobacco plant (Nicotiana tabacum L.). A randomised complete block design with three replications was applied in the field. Experiments were conducted on the fields of Soil and Water Resources Research Institute in Kyrklareli, Turkey. Three known stages of the plant were considered and a total of 14 irrigation treatments were applied in the experiments with K-326 Virginia tobacco cultivar. All the experimental treatments were irrigated at the same time as the control irrigated at each growth stage with water amount enough to replenish the water deficit in 0-90 cm soil layer, and three levels of water amount reductions (0, 40 and 60%) were done at each development stage. Results of the 3-years study showed that water stress imposed at earlier growth stages was influential mainly on plant growth, leaf formation and on the physiologically based parameters as net assimilation rate (NAR), leaf area index (LAI), absolute growth rate (AGR) and relative growth rate (RGR).