Detection of Parathyroid Hormone Using an Electrochemical Impedance Biosensor Based on PAMAM Dendrimers

Ozcan H. M., Sezginturk M. K.

BIOTECHNOLOGY PROGRESS, cilt.31, sa.3, ss.815-822, 2015 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 3
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1002/btpr.2060
  • Dergi Adı: BIOTECHNOLOGY PROGRESS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.815-822
  • Anahtar Kelimeler: parathyroid hormone, biosensor, PAMAM, electrochemical impedance spectroscopy, 12-mercapto dodecanoic, PRIMARY HYPERPARATHYROIDISM, MULTILAYER FILMS, GLANDS, IMMOBILIZATION, SPECTROSCOPY, IMMUNOASSAY, CALCIUM, SENSORS
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Hayır

Özet

This paper presents a novel hormone-based impedimetric biosensor to determine parathyroid hormone (PTH) level in serum for diagnosis and monitoring treatment of hyperparathyroidism, hypoparathyroidism and thyroid cancer. The interaction between PTH and the biosensor was investigated by an electrochemical method. The biosensor was based on the gold electrode modified by 12-mercapto dodecanoic (12MDDA). Antiparathyroid hormone (anti-PTH) was covalently immobilized on to poly amidoamine dendrimer (PAMAM) which was bound to a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) couple, self-assembled monolayer structure from one of the other NH2 sites. The immobilization of anti-PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscope techniques. After the optimization studies of immobilization materials such as 12MDDA, EDC-NHS, PAMAM, and glutaraldehyde, the performance of the biosensor was investigated in terms of linearity, sensitivity, repeatability, and reproducibility. PTH was detected within a linear range of 10-60 fg/mL. Finally the described biosensor was used to monitor PTH levels in artificial serum samples. (C) 2015 American Institute of Chemical Engineers