Upper Campanian-Maastrichtian Sayindere Formation, located in southeastern Turkey, composed of pelagic limestone which was deposited relatively deep marine. In this study, well samples of the Sayindere Formation were analyzed by Rock-Eval pyrolysis and the oil sample from this unit were analyzed by GC, and GC-MS to assess source rock characteristics and hydrocarbon potential. The TOC values of the Sayindere Formation samples range from 0.34 to 4.65 wt.% with an average of 1.14 wt.% and organic matter have good TOC value. Hydrogen Index (HI) values range from 407 mg HC/g TOC to 603 mg HC/g TOC and indicates Type II kerogen. T-max values are in the range of 434 - 442 degrees C and indicate early-mid mature stage. The Sayindere samples have fair to good hydrocarbon potential based on TOC contents, S2, and PY values. According to the HI versus TOC plot, most of the samples have good oil source. The oil sample contains predominant short-chain n-alkanes and plots in marine algal Type II field on a Pr/n-C17 versus Ph/n-C18 cross-plot indicating anoxic environment. Biomarker analysis shows that the deposition of oil source rock is carbonate-rich sediments.