Detection of some plant-specific retrotransposons in Gerze chicken by IRAP-PCR method

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Mercan L., Bülbül C. E., Bilgi F., Maraklı S.

IV. International Congress on Domestic Animal Breeding, Genetics and Husbandry - 2020 (ICABGEH-20), İzmir, Turkey, 12 - 14 August 2020, pp.152

  • Publication Type: Conference Paper / Summary Text
  • City: İzmir
  • Country: Turkey
  • Page Numbers: pp.152
  • Çanakkale Onsekiz Mart University Affiliated: Yes


Retrotransposons are a class of mobile genetic elements, constituting part of genome in different ratios. In this study, we aimed to identify Nikita and Sukkula retrotransposons known as specific to barley (Hordeum vulgare L.) in chicken genome and also the relationships between these retrotransposons and mobile genetic elements found in chicken genome. For this purpose, genomic DNAs were isolated from one of the endemic breeds of Turkey, Gerze chicken also named as Hacı Kadı. Retrotransposon movements were investigated by using retrotransposon-based molecular marker, IRAP. Although polymorphism was not found in Sukkula movement, polymorphism ratios of Nikita retrotransposon were 0-60%. Moreover, in silico analyses also showed closely relationships among these two retrotransposons and other chicken mobile sequences. With the results of this study, poultry biology could be better understood, and a new perspective could provide to understand the evolutionary relationships between plants and animals.