Glassy carbon electrode modified with poly-Neutral Red for photoelectrocatalytic oxidation of NADH


GİRAY DİLGİN D. , Gligor D., GÖKÇEL H. İ. , DURSUN Z., DİLGİN Y.

MICROCHIMICA ACTA, cilt.173, ss.469-476, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 173
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s00604-011-0582-7
  • Dergi Adı: MICROCHIMICA ACTA
  • Sayfa Sayıları: ss.469-476

Özet

A new approach is described for the photoelectrocatalytic oxidation of Reduced -Nicotinamide Adenine Dinucleotide (NADH). It is based on a glassy carbon electrode (GCE) modified with a film of poly-Neutral Red (poly-NR) that is obtained by electropolymerization. Electrochemical measurements revealed that the modified electrode displays electrocatalytic and photo-electrocatalytic activity towards oxidation of NADH. If irradiated with a 250-W halogen lamp, the electrode yields a strongly increased electrocatalytic current compared to the current without irradiation. Amperometric and photo-amperometric detection of NADH was performed at +150 mV vs. Ag/AgCl/KClsat and the currents obtained are linearly related to the concentration of NADH. Linear calibration plots are obtained in the concentration range from 1.0 mu M to 1.0 mM for both methods. However, the slope of the current-NADH concentration curve of the photo-electrocatalytic procedure was 2-times better than that obtained without irradiation.