Akademik Veri Yönetim Sistemi
INTERNATIONAL BALKAN AGRICULTURE CONGRESS, Çanakkale, Türkiye, 16 - 19 Kasım 2024, cilt.1, sa.1, ss.209, (Özet Bildiri)
Storage of mass production hosts of biological control agents is a common practice to ensure their availability. The purpose of this study was to determine the larval emergence from Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) eggs after storage in two different low temperatures and different storage periods. 1-day old E. kuehniella eggs were stored in 5°C and 8°C temperatures for 5, 10, 15, 20, 25, 30, 35 and 40 days in refrigerators. At the end of these periods, eggs were transferred to a climate chamber with 25±1°C temperature, %60-65 r.h. and 16:8 (L:D) photoperiod conditions. Number of emerging larvae was counted daily. The experiment was conducted with 10 replicates for each storage period with 50 E. kuehniella eggs in each replicate. For control, 10 replicates with 50 1- day old E. kuehniella egg were used. Data were analyzed with two-way ANOVA and TUKEY tests to determine the differences of larval emergence between storage temperatures and storage periods. For 5°C, difference between the larval emergence of storage periods was significant (p<0,05). At this temperature, the difference between control, 5-day and 10-day storage was not significant, while these storage periods were significantly higher than all the other periods. Similarly at 8°C, larval emergence of storage periods was significantly different between storage periods (p=0,05). At this temperature, the differences between control, 5-day and 10-day storages were also not significant, while they were significantly higher than other periods. The comparisons between the same storage periods at different temperatures showed that, there was no significant difference between temperatures at 5-day and 10-day storages. However, larval emergence numbers were significantly higher at 8°C for 15, 20, 25, 30, 35 and 40-day storage periods. Another difference between the temperatures was the larval emergence periods. Larvae emerged on the 2nd day after storage in 8°C, while emergence took 4 days after storage at 5°C. These results showed that, there wasn’t an important difference between unstored eggs, 5-day storage and 10-day storage at both temperatures. Thus, it is possible to store E. kuehniella eggs up to 10 days without any loss in larval emergence. However, larval emergence was higher if the storage period was 15 days or longer, at 8°C. Thus, temperature selection in short term storage is not important, while 8°C is a more suitable storage temperature for long term storage.
Keywords: Low temperature, storage, ephestia kuehniella, larval emergence, emergence rate