EFFECTS OF SUBCHRONIC EXPOSURE TO PHOSALONE ON OXIDATIVE STRESS AND HISTOPATHOLOGICAL ALTERATIONS IN COMMON CARP (Cyprinus carpio, L., 1758)


Kaya H. , ÇELİK E. Ş. , GÜRKAN M. , YILMAZ S. , Akbulut M.

JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, cilt.76, ss.853-864, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 76 Konu: 14
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1080/15287394.2013.823136
  • Dergi Adı: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
  • Sayfa Sayıları: ss.853-864

Özet

In this study, possible oxidative stress, biomarkers, and histopathological alterations were investigated in common carp (Cyprinus carpio, L., 1758) that were exposed to various phosalone concentrations in vivo. Fish were exposed to 0.15, 0.3, and 0.6 mg/L phosalone concentrations in a semistatic regime for 14 d. Biomarkers, including Na+, K+-ATPase, reduced glutathione (GSH), thiobarbituric acid-reactive substances (TBARS), and histopathological changes, were determined in gills, liver, and kidney tissues of fish. Sublethal phosalone concentrations produced lipid peroxidation and impairment in the antioxidant defense system by increasing reactive oxygen species (ROS) production. While GSH increased in all groups at d 7 compared to control, GSH fell significantly at medium and high doses at d 14. Reduced GSH levels were diminished in all tissues and a significant induction in lipid peroxidation (TBARS) was observed. Na+,K+-ATPase enzyme activity was significantly inhibited especially in gills. No histopathological effects were observed in the control group. Lamellar aneurysm, lamellar fusion, hyperplasia, epithelial lifting, and hemorrhages were observed in gill tissues exposed to phosalone. Histopathological effects in the liver tissues of fish exposed to phosalone were characterized by cytoplasmic vacuolation, congestion, hypertrophy, and nuclear degeneration. Hypertrophy, tubule degeneration, mononuclear cell infiltration, and dilation of glomerular capillaries were noted in kidney tissues exposed to phosalone. The results indicate that phosalone exposure adversely affected the health of the fish, attributable to oxidative stress.
In this study, possible oxidative stress, biomarkers, and histopathological alterations were
investigated in common carp (Cyprinus carpio, L., 1758) that were exposed to various
phosalone concentrations in vivo. Fish were exposed to 0.15, 0.3, and 0.6 mg/L
phosalone concentrations in a semistatic regime for 14 d. Biomarkers, including Na+,
K+-ATPase, reduced glutathione (GSH), thiobarbituric acid-reactive substances (TBARS), and
histopathological changes, were determined in gills, liver, and kidney tissues of fish. Sublethal
phosalone concentrations produced lipid peroxidation and impairment in the antioxidant
defense system by increasing reactive oxygen species (ROS) production.While GSH increased
in all groups at d 7 compared to control, GSH fell significantly at medium and high doses at d
14. Reduced GSH levels were diminished in all tissues and a significant induction in lipid peroxidation
(TBARS) was observed. Na+,K+-ATPase enzyme activity was significantly inhibited
especially in gills. No histopathological effects were observed in the control group. Lamellar
aneurysm, lamellar fusion, hyperplasia, epithelial lifting, and hemorrhages were observed in
gill tissues exposed to phosalone. Histopathological effects in the liver tissues of fish exposed
to phosalone were characterized by cytoplasmic vacuolation, congestion, hypertrophy, and
nuclear degeneration. Hypertrophy, tubule degeneration, mononuclear cell infiltration, and
dilation of glomerular capillaries were noted in kidney tissues exposed to phosalone. The
results indicate that phosalone exposure adversely affected the health of the fish, attributable
to oxidative stress.