In Silico Genome-Wide Identification and Analysis of Na+/H+ Antiporter (NHX) Genes in Solanum lycopersicum

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Çavuşoğlu E., Sarı U., Tiryaki İ.

SOL International Online Meeting 2020, 9 - 11 November 2020, pp.94

  • Publication Type: Conference Paper / Summary Text
  • Page Numbers: pp.94
  • Çanakkale Onsekiz Mart University Affiliated: Yes


Salinity which is spreading throughout the world is one of the most important abiotic stress factors limiting the agricultural productivity worldwide. The Na+/H+ antiporter (NHX) genes play a considerable role during plant growth and development in tolerance to salt stress. The objective of this study is to identify NHX genes using version SL4.0 and version ITAG4.0 of tomato genome. Seven putative NHX genes distributed on chromosome 1, 4, 6, and 10 were identified. While SlNHX1, -2, -3, -4 and -6 genes were localized on Vacuole, SlNHX7 and SlNHX8 were localized on plasma membrane and Plasma Membrane-Vacuole, respectively. SlNHX1, -2, 3-, -4, -6 proteins have amiloride-binding site [FFIYLLPPI] which is located in transmembrane helix domain-3 and is known an inhibitor of Na+/H+ exchanger activity and characteristic feature of Vacuole class NHX proteins. SlNHX4 and SlNHX7 hypothetically interact with CBL-interacting protein kinases (CIPK) based on Protein-protein interaction (PPI) prediction analysis. The High-throughput gene expression data analysis which was retrieved from TomExpress database and SolGenomics project. Whereas SlNHX3 gene only expressed in unopened flower, SlNHX2, -4 and SlNHX1, -6 genes showed the highest expression levels in root and mature green fruit stage, respectively. The tissue specific gene expression levels treated with salt shock stress at different salt concentrations will be performed using qRT-PCR after plants reach at targeted development stage.