Method validation for the analysis of pesticide residues in grain by thin-layer chromatography

TİRYAKİ O.

ACCREDITATION AND QUALITY ASSURANCE, vol.11, no.10, pp.506-514, 2006 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 10
  • Publication Date: 2006
  • Doi Number: 10.1007/s00769-006-0171-3
  • Journal Name: ACCREDITATION AND QUALITY ASSURANCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.506-514
  • Keywords: method validation, TLC detection method, precision, calibration, VEGETABLES, LINEARITY, FRUITS, TLC
  • Çanakkale Onsekiz Mart University Affiliated: No

Abstract

Method validation is an important requirement in the practice of pesticide residue analysis and is the process of verifying that a method is fit for its purpose. To make a correct decision on the validity of the method, the following method performance parameters have to be taken into consideration: scope, specificity, limit of detection, limit of quantification, linear range, accuracy, precision, repeatability, reproducibility, recovery, ruggedness and robustness. The goal of this study was to validate previously adapted thin-layer chromatographic methods for the pesticide residue analysis in grain. Confirmation of validation parameters for some compounds was also performed by gas chromatographic analysis.