Method validation for the analysis of pesticide residues in grain by thin-layer chromatography


TİRYAKİ O.

ACCREDITATION AND QUALITY ASSURANCE, vol.11, no.10, pp.506-514, 2006 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 10
  • Publication Date: 2006
  • Doi Number: 10.1007/s00769-006-0171-3
  • Journal Name: ACCREDITATION AND QUALITY ASSURANCE
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.506-514
  • Keywords: method validation, TLC detection method, precision, calibration, VEGETABLES, LINEARITY, FRUITS, TLC

Abstract

Method validation is an important requirement in the practice of pesticide residue analysis and is the process of verifying that a method is fit for its purpose. To make a correct decision on the validity of the method, the following method performance parameters have to be taken into consideration: scope, specificity, limit of detection, limit of quantification, linear range, accuracy, precision, repeatability, reproducibility, recovery, ruggedness and robustness. The goal of this study was to validate previously adapted thin-layer chromatographic methods for the pesticide residue analysis in grain. Confirmation of validation parameters for some compounds was also performed by gas chromatographic analysis.