Research Information System
3rd International Conference on Applied Engineering and Natural Sciences (ICAENS 2022), Konya, Turkey, 20 - 23 July 2022, vol.1, no.1, pp.335-340
Drought stress, known to have a limiting effect on crop growth and yield, adversely affects
wheat production worldwide due to global climate change. Osmolytes such as glycine-betaine (GB) play
an osmoprotectant in the scavenging of reactive oxygen species formed under osmotic stress conditions
such as drought stress and are naturally synthesized in some plants under stress conditions. It is known that
GB has a vital role in the plant's abiotic stress resistance, including growth, photosynthesis, maintenance of
membrane integrity, and oxidative defense. In this study, some physiological (shoot and root length,
biomass, specific leaf area (SLA), total chlorophyll content (SPAD) and relative water content (RWC)) and
biochemical (hydrogen peroxide (H2O2) amount and histochemical staining (H2O2)) changes caused by
exogenous GB treatment in two wheat cultivars (drought-sensitive Selçuklu, drought-resistant Demir-2000)
grown in Turkey with different drought tolerance were investigated. Accordingly, drought stress was
initiated in 20-day-old wheat seedlings sprayed with 5 mM GB at 24 hours following the application and
at the end of 7 days, leaf and root sampling were done.
Compared to Demir-2000, drought stress increased the amount of H2O2 and decreased other physiological
parameters in Selçuklu. GB treatment reduced the drought-related damage in both genotypes. As a result,
it was determined that drought damage in the susceptible genotype was improved at the resistant genotype
level with GB treatment, except for the amount of H2O2.