In this research, high molecular weight glutenin (HMWG) subunit composition, found on Glu-1 locus of 41 genotypes selected according to grain yield, yield components and some quality properties from bread wheat landraces collected from Eastern Turkey, has been investigated by using SDS-PAGE method. Of the bread wheat pure lines, nine Glu-1 locus alleles have been determined in genotypes. While only null allele (c) on Glu-A1 locus has been determined, on Glu-B1 locus 7+8 subunit (b) in 35 pure lines (85.4%), 13+16 subunit (f) in 2 pure lines (4.9%), subunits 7 (a), 14+15 (h), 17+18 (i) and 6+8 (d) (2.4%) have been determined in only one genotype. On Glu-D1 locus allele a in 34 pure lines (82.9%) and allele d in 7 pure lines (17.1%) have been determined. In this research in which 7 different HMWG subunit combination were determined, the most common group found in 32 pure lines (78.0%) was n, 7+8 and 2+12. Of the genotypes, only 5 pure lines (12.2%) have taken 8 quality score value whereas the quality score value of 36 pure lines (87.8%) has been determined changing between 4 and 6.