The methanol extracts obtained from endemic Scrophulariaceae members Verbascum protractum Fenel ex Tchihat., Verbascum bellum Hub.- Mor., Verbascum dalamanicum Hub.- Mor., Scrophularia mersinensis Lall, Scrophularia cryptophila Boiss. & Heldr., Pedicularis olympica Boiss., and Veronica lycica E. Lehm. have been investigated for their antimicrobial activity. Antimicrobial activity was determined with Escherichia coli ATCC 11230, Staphylococcus aureus ATCC 6538P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis CCM 2067, Listeria monocytogenes ATCC 15313, Micrococcus luteus CCM 169, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403, and Kluyveromyces fragilisATCC 8608 by the disk diffusion method. The extracts of all Scrophulariaceae members used in this study had strong antimicrobial activity against the Gram- positive bacteria and yeast cultures.