Akademik Veri Yönetim Sistemi
Journal of Drug Delivery Science and Technology, cilt.122, 2026 (SCI-Expanded, Scopus)
2-Methacryloyloxyethyl phosphorylcholine (MPChl) monomer is advantageous for preparing micro/nano sized gels (particles) for use particularly in biomedical applications, due to its bioinert, biocompatible, and nontoxic properties. Herein, preparation of hydrolytically degradable and biocompatible poly(2-Methacryloyloxyethyl phosphorylcholine) (p(MPChl)) microgels and their use in drug delivery system were reported. The p(MPChl) microgels were synthesized via dispersion polymerization using bis[2-(methacryloyloxy)ethyl]phosphate (BMEP) crosslinker and 2,2′-azobis (2-methylpropionitrile) (AIBN) initiator. The synthesized spherical p(MPChl) microgel had sizes about 1299 ± 225 nm (by DLS analysis) are suitable for biomedical uses. The hydrolytic degradation of p(MPChl) microgels was investigated in various body fluid environments, at pH 1.0, 7.4, and 9.0. The p(MPChl) microgels are highly degradable at physiological pH 7.4. Blood compatibility tests of p(MPChl) microgels revealed that they are excellent hemocompatible materials with hemolysis% (H%) of 0.65% and blood coagulation index% (BCI%) of 91 ± 5 even at high microgel concentrations, 1000 μg/mL. A model antibiotic, amoxicillin (AmoxT) was loaded into p(MPChl) microgels at relatively high amount, 813.06 μg/mg from aqueous solution, and released more than 50% of the loaded drug up to 3 days in linear release profile whereas negligible release of AmoxT was observed at solutions with pH 1 and 9.0. The hydrolytic degradation ability and drug release behavior of the p(MPChl) microgels showed parallel results. Moreover, the cytotoxicity studies of AmoxT-loaded p(MPChl) microgels at 500 μg/mL concentrations were determined to be non-toxic for L929 fibroblast cells. Interestingly, even at a high concentration of AmoxT-loaded p(MPChl) microgels e.g.1000 μg/mL showed relatively low toxicity, and 82 ± 4% cell viability. These findings suggest that p(MPChl) microgels are promising and safe candidates for hydrolytically degradable, hemocompatible and cytocompatible applications in vivo drug delivery systems.