Glucose monitoring by a biosensor based on mercury thin film electrodes
ANALYTICAL LETTERS, cilt.41, sa.6, ss.1074-1082, 2008 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 41 Sayı: 6
- Basım Tarihi: 2008
- Doi Numarası: 10.1080/00032710801978483
- Dergi Adı: ANALYTICAL LETTERS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1074-1082
- Anahtar Kelimeler: biosensor, Clark electrode, glucose, glucose oxidase, mercury thin film, ANODIC-STRIPPING VOLTAMMETRY, GLASSY-CARBON ELECTRODES
- Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet
Özet
Mercury film electrodes consist of a thin film of mercury deposited on an electrode surface ( typically glassy carbon) by reduction of a mercury (II) salt in solution. The surface area/volume ratio is larger for the mercury film electrode, and this electrode is more stable than mercury drop electrode, which allows a faster stirring rate to be used in the deposition step. An enzyme electrode is described, based on glucose oxidase immobilized by gelatin and glutaraldehyde and held over a glassy carbon electrode coated with a thin mercury film. This biosensor responds fast and linearly to glucose in a wide concentration range, which is significant because monitoring of glucose levels is a critical component of diabetes care. Certain optimization and characterization studies were carried out. Average value, standard deviation (SD), and variation coefficient ( CV) were calculated with the help of the repeatability studies. Finally, glucose content of human blood samples was monitored with the help of the biosensor presented.