Electrooxidation of Phenazopyridine Hydrochloride and its Voltammetric and HPLC Determination in Human Urine and Tablet Dosage Form


YAĞMUR S. , YILMAZ S. , Sadikoglu M., SAĞLIKOĞLU G. , YILDIZ M. , YENGİN Ç., ...Daha Fazla

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, cilt.8, ss.6818-6828, 2013 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 8 Konu: 5
  • Basım Tarihi: 2013
  • Dergi Adı: INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
  • Sayfa Sayıları: ss.6818-6828

Özet

The electrochemical oxidation of phenazopyridine hydrochloride (PAP) was investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques at ultra trace graphite electrode (UTGE). PAP exhibited a diffusion controlled process which is pH dependence. The dependence of the current and potential on pH, the concentration and nature of buffer, and scan rate was investigated to optimize the experimental conditions for the determination of PAP. It was found that the optimum buffer for the determination of PAP is 0.1 NaOH solutions with the pH 12.97. In the concentration range from 6.0x10(-8) to 1.0x10(-6) M, the current measured by DPV present a good linear property as a function of the concentration of PAP. Limit of detection (LOD) and limit of quantification (LOQ) were obtained as 8.1x10(-10) and 2.7x10(-9) M, respectively. PAP was determined in human urine and tablet dosage form. Precision and accuracy of the developed technique were checked by recovery studies in spiked urine and tablet dosage form. In addition, for the comparison, high performance liquid chromatography (HPLC) technique was applied for the determination of PAP in the same samples.