An amperometric inhibitor biosensor for the determination of reduced glutathione (GSH) without any derivatization in some plants

Sezginturk M. K., DINCKAYA E.

BIOSENSORS & BIOELECTRONICS, cilt.19, sa.8, ss.835-841, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 8
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1016/j.bios.2003.08.012
  • Dergi Adı: BIOSENSORS & BIOELECTRONICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.835-841
  • Anahtar Kelimeler: ascorbate oxidase, glutathione determination, GSH, GSSG, inhibitor biosensors, PERFORMANCE LIQUID-CHROMATOGRAPHY, FLOW-INJECTION DETERMINATION, QUANTITATIVE-DETERMINATION, ELECTROCHEMICAL DETECTION, GAMMA-GLUTAMYLCYSTEINE, OXIDIZED GLUTATHIONE, BIOLOGICAL THIOLS, OXYGEN-TOXICITY, HUMAN PLASMA, SEPARATION
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Hayır

Özet

In this study, an amperometric biosensor based on cucumber tissue homogenate was developed for the determination of glutathione. Cucumber (Cucumis sativus L.) tissue homogenate was used as the biological material. The cucumber tissue homogenate was cross-linked with gelatine using glutaraldehyde and fixed on a pretreated teflon membrane. The principle of the measurements was based on the determination of the decrease in the differentiation of oxygen level which had been caused by the inhibition of ascorbate oxidase in the biological material by glutathione. Determinations were carried out by standard curves which were obtained by the measurement of the decrease in the consumed oxygen level related to glutathione concentration.