The aim of the present study was to compare the cyclic fatigue resistances of HyFlex EDM (HEDM), WaveOne Gold (WOG), Reciproc Blue (RB), and 2Shape (TS) NiTi systems having different metallurgic properties. HEDM, WOG, RB, and TS instruments were rotated in artificial canals which were made of stainless steel with an inner diameter of 1.5mm, 45 degrees, and 90 degrees angles of curvatures and a radius of curvature of 5mm until fracture occurred, and the time to fracture (TTF) was recorded in seconds. Also, number of cycles to fracture was calculated (NCF). Data were statistically analyzed using Kruskal-Wallis and t test. The statistical significance level was set at P<0.05. When comparing the TTF of all the instruments tested in the artificial canal with 45 degrees curvature, RB had statistically the highest cyclic fatigue resistance followed by HEDM and TS (P<0.05), while the WOG showed the lowest cyclic fatigue resistance (P<0.05). In artificial canal with 90 degrees curvature, RB showed a significantly higher cyclic fatigue resistance than the other tested files (P<0.05), while there was no significant difference among the TS, WOG and HEDM groups (P>0.05). When the NCF values were taken into consideration, HEDM reported a significantly higher cyclic fatigue resistance than TS in both canal curvatures analyzed (P<0.05). Within the limitations of the present study, RB NiTi files showed statistically higher cyclic fatigue resistance in artificial canals with 45 degrees and 90 degrees than the other NiTi files tested.