A Biosensor for the Determination of beta-galactosidase Activity: A Different Viewpoint on Biosensors
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.39, sa.5, ss.281-288, 2011 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 39 Sayı: 5
- Basım Tarihi: 2011
- Doi Numarası: 10.3109/10731199.2011.560119
- Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.281-288
- Anahtar Kelimeler: beta-galactosidase, glucose oxidase, biosensor, enzyme activity, electropolymerization, ALKALINE-PHOSPHATASE, ESCHERICHIA-COLI, IN-VIVO, SURFACES, ENZYME, LABEL, WATER
- Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet
Özet
beta-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor beta-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. beta-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for beta-galactosidase detection was in the range of 0.0118 U mL(-1) to 0.47 U mL(-1) and a shorter response time of similar to 50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of beta-galactosidase activity.