In this study, the concentrations of elements in muscle, liver and cartilage tissues of spiny dogfish (Squalus acanthias) were investigated. Spiny dogfish caught from the Marmara Sea at three different times of the year (October 2008, January and April 2009) were purchased from sea products factories (exporter) in Canakkale (Turkey). The results showed that the element accumulations were higher in liver than the cartilage and muscle tissues in all three months. Al, Fe and Zn concentrations in all examined tissues were remarkably higher than the other elements. However, all the elements (especially Al, Cd, Pb) were below the Turkish legislation and European Commission legal limits for fish muscle, cartilage and liver. Therefore, we can conclude that Squalus acanthias from Marmara Sea (Turkey) are safe for human consumption.