Effects of chromatin remodeling complexes on trehalose accumulation in yeast Saccharomyces cerevisiae


Turgut Genç T., Akıncı N.

7. International Molecular Biology and Biotechnology Congress (MOLBIOTECH), Konya, Turkey, 25 - 27 April 2018, vol.1, no.1, pp.1

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Konya
  • Country: Turkey
  • Page Numbers: pp.1

Abstract

Trehalose has many important physiological roles in Saccharomyces cerevisiae, such as energy and carbon source, reserve carbonhydrate and metabolic regulator in yeast. Furthermore many studies have shown that trehalose is accumulated under stress conditions like heat stress, ethanol stress, nutrientional starvation, desiccation and osmotic stress. In other words, trehalose contentof cell is an indicator of stress tolerance. The genes involved in trehalose synthesis and breakdown is thightly regulated in both transcriptional and protein level. Chromatin remodelers have a role in transcriptional regulation. SWI/SNF chromatin remodeling complex mobilizes nucleosomes, and SAGA complex acts as a coactivator to recruit the TATA-binding protein to the TATA. SWR1 complex replaces the canonical histone H2A with the variant H2A.Z. In this study we investigated the effects of SWR1, SAGA and SWI/SNF chromatin remodelers on the trehalose accumulation by means of enzymatic assay. In our researh, the exponentional growing cells of Δswr1, Δspt7, Δsnf2 mutant yeast strains, the essential subunits of these complexes, respectively, and their wilde type strain were used. Trehalose contents of Δswr1 and Δspt7 yeast cells were similar to wild type. But trehalose accumulation of Δsnf2 mutant yeast cells was 20-25 fold higher than wild type yeast cells. These resuls showed that SWI/SNF chromatin remodeling complex is essential for regulation of trehalose metabolism.