Heavy Metal Biosorption and Plasmid Profiles of Heavy Metal Resistance Bacteria isolated from Soil Samples of Thermal power, Canakkale, Turkey


DÜLGER G., AKI C.

RESEARCH JOURNAL OF BIOTECHNOLOGY, cilt.12, ss.42-48, 2017 (ESCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 12 Konu: 9
  • Basım Tarihi: 2017
  • Dergi Adı: RESEARCH JOURNAL OF BIOTECHNOLOGY
  • Sayfa Sayıları: ss.42-48

Özet

The aim of this study is to investigate the isolation, characterization, determination of plasmid contents and metal absorption capacities of heavy metal-resistant bacteria from soil samples of thermal power. Samples were collected from soil in the area of thermal power. To define the resistance of heavy metal, metal solutions of ZnCl2, FeCl3 6 center dot H2O, Pb(NO3)(2) and CuSO4 in varying concentrations were added to culture media by using MIC method. The bacteria of highest MIC level were defined through VITEK 2 system and their plasmid profiles were determined. The metal biosorption capacities of two isolates were defined through ICP-AES. In total, 37 heavy metal resistant bacteria were isolated from soil samples. Results showed that 15 of them contained plasmid DNA. Finally, biosorption experiments with two isolates with the highest metal resistance were performed. While the metal having the highest growing speed of biosorption among Fe, Zn, Pb and Cu metals which were used in this study for Bacillus mycoides (isolate 26) is Zn, it is followed by Pb, Fe and Cu orderly. While the metal having the highest growing speed of biosorption for Enterobacter cloacea complex (isolate 10) is Pb, it is followed by Zn, Fe and Cu orderly.