Quantitative Analysis of a Promising Cancer Biomarker, Calretinin, by a Biosensing System Based on Simple and Effective Immobilization Process

Asav E., Sağıroğlu A., Sezginturk M. K.

ELECTROANALYSIS, cilt.28, sa.2, ss.334-342, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 2
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1002/elan.201500324
  • Dergi Adı: ELECTROANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.334-342
  • Anahtar Kelimeler: Calretinin, epichlorohydrin, cancer biomarker, immunosensor, Electrochemical Impedance Spectroscopy, SELF-ASSEMBLED MONOLAYERS, SURFACE-PLASMON RESONANCE, HYDROXYL-GROUPS, PROTEIN, GOLD, EXPRESSION, ACTIVATION, IMMUNOHISTOCHEMISTRY, ADSORPTION, DIAGNOSIS
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Hayır

Özet

Calretinin (CAL) is calcium binding protein, and its levels in blood and cerebrospinal fluids are increased, since its expression is increased various cancer types. A novel biosensor system fabricated by immobilization of a specific antibody to CAL, anti-Calretinin (anti-CAL), onto a gold electrode surface via an effective covalent binding method using mercaptohexanol, epichlorohydrin, and ethanolamine was reported for the sensitive, selective, and accurate analysis of CAL. The proposed biosensor showed a linear calibration range between 1 ng/mL and 5 ng/mL. LOD and LOQ values were determined as 0.11 ng/mL and 0.38 ng/mL, respectively. The standard deviation related to the reproducibility of the new biosensor system was calculated as 3.95%. Lastly, in order to state the applicability of the biosensor to early diagnosis of CAL in practice, artificial serum samples spiked with CAL have been analyzed by the proposed biosensor.