Single-stranded DNA (ssDNA) Aptamer targeting SipA protein inhibits Salmonella Enteritidis invasion of intestinal epithelial cells

Shatila F., Yalcin H. T., ÖZYURT C., EVRAN S., Cakir B., YAŞA İ., ...More

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.148, pp.518-524, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 148
  • Publication Date: 2020
  • Doi Number: 10.1016/j.ijbiomac.2020.01.132
  • Journal Name: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.518-524
  • Keywords: Aptamer, Salmonella invasion, Salmonella invasion protein A, IN-VITRO SELECTION, MYCOBACTERIUM-TUBERCULOSIS, ANTIBIOTIC-RESISTANCE, TYPHIMURIUM, ACTIN, BIND, BIOFILM, ENTRY
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

Salmonella Enteritidis is an important pathogen that can invade the intestinal cells of its host causing salmonellosis. SipA protein, an effector protein secreted by T3SS, maintains invasion of host cells more efficient. Thus, inhibitory aptamers against SipA protein were developed using magnetic bead-based Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method. The enriched sequences were obtained after 9 SELEX rounds. Among which, an aptamer namely Apt17 displayed Kd values equivalent to 114.9 and 63.4 nM at 27 degrees C and 37 degrees C, respectively. The effect of Apt17 on adhesion and invasion of Caco-2 cells by the tested strains was determined. While the adhesion and invasion of Salmonella Enteritidis TM 6 were inhibited by 70% and 37.7%, those of Salmonella Enteritidis TM 68 were inhibited by 45.71% and 39.5% respectively. These results represent a corner stone for future studies that could aim to develop putative inhibitors against Salmonellosis. (C) 2020 Elsevier B.V. All rights reserved.