Preparation of hyaluronic acid and copolymeric hyaluronic acid: sucrose particles as tunable antibiotic carriers


Sahiner N., Suner S., Ayyala R. S.

JOURNAL OF POLYMER RESEARCH, vol.27, no.7, 2020 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 7
  • Publication Date: 2020
  • Doi Number: 10.1007/s10965-020-02168-4
  • Journal Name: JOURNAL OF POLYMER RESEARCH
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Keywords: Copolymeric hyaluronic acid-sucrose, Micro, nanoparticle, Sustainable antibiotic release, Antimicrobial material, DRUG-DELIVERY, MICROPARTICLES, CIPROFLOXACIN, NANOPARTICLES, MICELLES

Abstract

Hyaluronic acid (HA) and copolymeric HA:Sucrose (HA:Suc) particles were synthesis with two different crosslinkers (Xs), divinyl sulfone (DVS) and glycerol diglycidyl ether (GDE) in 0.5-20 mu m size range. The sustenance potential of HA:Suc particles in bacterial growth media was examined with time, up to 72 h incubation time in the cultivation of various gram-negative (GN) and gram-positive (GP) bacterial strains. Furthermore, ciprofloxacin, model drug was loaded into HA and HA:Suc particles few times by chemical conjugations; e.g., 1st and 2nd chemical conjugations and named as 1C and 2C, respectively. To increase the drug loading amount into HA-based particles, 2C particles was placed in drug solution for physical adsorption (2C + A). Therefore, drug carrier capacity of HA-based particles was enhanced almost 6.5-fold with the highest ciprofloxacin release from HA particles was 43.47 +/- 2.36 mg/g within 120 h. The antimicrobial susceptibility studies revealed that ciprofloxacin-loaded copolymeric HA:Suc particles with 2C + A loading process has the highest antimicrobial effects. The minimum inhibition concentration (MIC) against E. coli, P. aeruginosa, S. aureus, and B. subtilis strains were found to range between 0.25-1 mg/mL, and an increasing inhibition trend was observed by increasing the incubation time up to 72 h due to linear drug release profile from HA particles.