Research Information System
Karadeniz Zirvesi 6. Uluslararası Uygulamalı Bilimler Kongresi, Sinop, Turkey, 5 - 06 June 2021, pp.58-64, (Full Text)
Saccharomyces cerevisiae yeast cells have been used as a model organism in recent years to
understand the mechanisms of aging which is similar in yeast and higher eukaryotes. There are
two types of aging in S. cerevisiae yeast cells: replicative and chronological aging. The
replicative life span represents the number of mitotic division performed by a single yeast cell,
while the chronological life span begins at the post-diauxic stage and represents the duration of
survival over the stationary phase. In recent years, the investigation and discovery of natural,
plant-based geroprotectors have gained importance. In our study, the mahaleb kernel extracts
(Prunus mahaleb L.) were used for analyzing the effects on yeast growth inhibition. The used
extraction solvents were hexane, acetone, methanol and water. Growth inhibition tests were
done in different concentrations of each extract using a microplate reader. The results showed
that acetone and hexane concentrations above 0.1M and methanol concentrations above 1.0M
prevented the growth of yeast cells. In addition, it was determined that the growth of yeast cells
was not inhibited, but activated in all water extract concentrations used. According to these
results, it is highly probable that the water extracts of the mahaleb kernel have a geroprotective
property.