THE EFFECTS OF MAHALEB KERNEL EXTRACTS ON THE GROWTH OF SACCHAROMYCES CEREVISIAE YEAST CELLS


Creative Commons License

Turgut Genç T., Sargın M.

Karadeniz Zirvesi 6. Uluslararası Uygulamalı Bilimler Kongresi, Sinop, Turkey, 5 - 06 June 2021, pp.58-64

  • Publication Type: Conference Paper / Full Text
  • City: Sinop
  • Country: Turkey
  • Page Numbers: pp.58-64
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

Saccharomyces cerevisiae yeast cells have been used as a model organism in recent years to

understand the mechanisms of aging which is similar in yeast and higher eukaryotes. There are

two types of aging in S. cerevisiae yeast cells: replicative and chronological aging. The

replicative life span represents the number of mitotic division performed by a single yeast cell,

while the chronological life span begins at the post-diauxic stage and represents the duration of

survival over the stationary phase. In recent years, the investigation and discovery of natural,

plant-based geroprotectors have gained importance. In our study, the mahaleb kernel extracts

(Prunus mahaleb L.) were used for analyzing the effects on yeast growth inhibition. The used

extraction solvents were hexane, acetone, methanol and water. Growth inhibition tests were

done in different concentrations of each extract using a microplate reader. The results showed

that acetone and hexane concentrations above 0.1M and methanol concentrations above 1.0M

prevented the growth of yeast cells. In addition, it was determined that the growth of yeast cells

was not inhibited, but activated in all water extract concentrations used. According to these

results, it is highly probable that the water extracts of the mahaleb kernel have a geroprotective

property.