In this study, the Hipparcos and ASAS light curves of the eclipsing binary V399 Pup have been analyzed using the Wilson-Devinney program. The light curve analyses have found that V399 Pup is a detached binary system with eccentric orbit. The apsidal motion parameters of the system have been obtained from the O-C curve analyses. The system has been revealed to have an orbital eccentricity of e = 0.145 +/- 0.006, the apsidal motion rate has been obtained omega = 0.0385 +/- 0.0069 deg cycle(-1), and it corresponds to an apsidal motion period of U = 100.2 +/- 18.0 years. (C) 2014 Elsevier B.V. All rights reserved.