A novel electrochemical immunosensor based on ITO modified by carboxyl-ended silane agent for ultrasensitive detection of MAGE-1 in human serum

Gundogdu A., AYDIN E. B., SEZGİNTÜRK M. K.

ANALYTICAL BIOCHEMISTRY, cilt.537, ss.84-92, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 537
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.ab.2017.08.018
  • Dergi Adı: ANALYTICAL BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.84-92
  • Anahtar Kelimeler: Melanoma-associated antigen 1, Electrochemical immunosensor, Indium tin oxide, Carboxyethylsilanetriol, ENDOTHELIAL GROWTH-FACTOR, SELF-ASSEMBLED MONOLAYER, TIN OXIDE ELECTRODE, LABEL-FREE, CANCER/TESTIS ANTIGENS, CANCER, BIOSENSOR, GOLD, GRAPHENE, IMMOBILIZATION
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

A new, low-cost electrochemical immunosensor was developed for rapid detection of Melanoma associated antigen 1 (MAGE-1), a cancer biomarker. The fabrication procedure of immunosensor was based on the covalent immobilization of anti-MAGE-1, biorecognition molecule, on ITO electrode by carboxyethylsilanetriol (CTES) monolayer. The biosensing MAGE-1 antigen was monitored by using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) technique. Apart from these techniques, single frequency impedance (SFI) was used for investigation of antibody-antigen interactions. Scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) were utilized for characterization of the proposed biosensor. To fabricate highly sensitive, good stability immunosensor, some parameters were optimized. Under optimal conditions, the developed electrochemical immunosensor for MAGE-1 exhibited a dynamic range of 4 fg/mL and 200 fg/mL with a low detection limit of 130 fg/mL. It had acceptable repeatability (5.05%, n = 20) and good storage stability (3.58% loss after 10 weeks). Moreover, this electrochemical immunosensor has been successfully applied to the determination of MAGE-1 in human serum samples. (C) 2017 Elsevier Inc. All rights reserved.