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2. International Rating Academy Congress on HOPE, Çanakkale, Turkey, 19 - 21 April 2018, vol.1, no.1, pp.1
Trehalose is a non-reducing disaccharide, present in many organisms including yeast. Many research demonstrated that the higher trehalose accumulation in the yeast cell, the higher tolerance against various environmental stresses. Thus, the genes encoding enzymes involved in trehalose synthesis and hydrolysis were also upregulated under stress conditions. Chromatin plays a crucial role in the control of nuclear processes including transcription. Two general types of chromatin- modifying complexes have been described. One type covalently modifies histones by acetylation, methylation or phosphorylation like SAGA complex, the second type is the ATP-dependent nucleosome-remodeling complexes that noncovalently modify chromatin, like SWI/SNF and SWR1 complex. SWI/SNF as well as SAGA are recruited to acetylated histones for chromatin remodeling. SWR1 complex is necessary for the incorporation of the histone variant H2AZ into chromatin. In this study, we investigated the effect of SWI/SNF, SWR1 and SAGA complex on trehalose accumulation under osmotic stress conditions. For this purpose Δsnf2, Δswr1 and Δspt7 yeast mutants and their isogenic wild type yeast strain were used to determine trehalose accumulation enzymatically. In Δsnf2 mutants, the accumulation of trehalose was 4-times higher than wild type both in normal and stress conditions. In Δswr1 mutants, trehalose content was 4-fold lower than wild type under stress conditions, and in Δspt7 mutants trehalose was accumulated very low levels than wild type in both conditions. These results show that all three chromatin remodellers have arole in trehalose metabolism in different ways. SWI/SNF comlex has a role in down-regulation but SWR1 and SAGA complex have a role in up-regulation of trehalose metabolism.