Aim: Nurses play a crucial role in providing holistic care. Moral sensitivity can help nurses at this point. The objective of this study was to investigate the perception of spiritual care and moral sensitivity of senior nursing students receiving education in five different regions of Turkey. Method: This study used a multicenter, descriptive and cross-sectional survey design. Data were collected with the Spirituality and Spiritual Care Rating Scale and the Moral Sensitivity Questionnaire in the research. A total of 590 senior nursing students, studying in five different universities in five different regions of Turkey, participated in this research. Results: The mean total score of students on the Spirituality and Spiritual Care Rating Scale was 4.06±0.70 and Moral Sensitivity Questionnaire was 5.44±1.12. There was a significant relationship between the mean total scores on the Spirituality and Spiritual Care Rating Scale and the Moral Sensitivity Questionnaire (p<0.05). Conclusion: Effective spiritual care can be provided by nurses who show high moral sensitivity. It is recommended to benefit from different and innovative educational practices related to spirituality and moral sensitivity in nursing education, and to conduct interventional and longitudinal studies on these issues.