Self-assembled silibinin-containing phosphazene/cystamine hybrid nanospheres as biodagradable dual-drug carriers with improved anticancer activity on a breast cancer cell line

Ozsoy F., Cömert Önder F., Ilgın P., Özay H., Önder A., Özay Ö.

MATERIALS TODAY COMMUNICATIONS, vol.37, pp.1-11, 2023 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 37
  • Publication Date: 2023
  • Doi Number: 10.1016/j.mtcomm.2023.107116
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex, INSPEC
  • Page Numbers: pp.1-11
  • Çanakkale Onsekiz Mart University Affiliated: Yes


In this study, pH and redox sensitive biodegradable PPZs@Silibinin polymeric nanospheres were synthesized by

the polymerization reaction of Hexachlorocyclotriphosphazene (HCCP), Cystamine dihydrochloride (CA) and the

anticancer drug Silibinin as monomers. Later designed as a therapeutic system, this nano drug-carrier was also

loaded with Doxorubicin (Dox), a type of cancer drug. As a result, a drug self-delivery system (DSDS) was

developed, which had the property of biodegradation in tumor tissues thanks to the redox active disulfide bonds

in its structure from pH-sensitive nanospheres containing two anticancer drugs. Biodegradation-associated in

vitro release of cancer drug (Dox) from the Dox loaded PPZs@Silibinin nano drug-carrier was performed in the

presence of glutathione (GSH) at pH 6.5 and pH 7.4. While 93.06% Dox release was observed in pH 6.5 medium

containing 10 mM GSH in 24 h, this value was 56.60% in pH 7.4 + 10 mM GSH medium. In addition, clonogenic

analysis and cytotoxicity studies were performed on the MDA-MB-231 cell line to determine the anticancer

properties of PPZs@Silibinin loaded with Dox. It was determined that PPZs@Silibinin@Dox nanospheres containing

20.5 nM Dox for 0.75 μL are highly effective in MDA-MB-231 cells than free Dox