Improving the application of microbial lipase by bio-imprinting at substrate-interfaces

Yilmaz E.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.18, no.1, pp.37-40, 2002 (SCI-Expanded) identifier identifier


Lipases have bio-imprinted with common substrate-interfaces and interesterification activities compared with amphiphile bio-imprinted counterparts. Bio-imprinting has yielded a 3.5- to 4.5-fold activity enhancement. Solvent-free medium was equally effective as hexane medium. Water addition erased the bio-imprinting effect. Bio-imprinting caused rate acceleration in the interesterification reaction and increased thermostability of the enzyme.