Improving the application of microbial lipase by bio-imprinting at substrate-interfaces
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, cilt.18, sa.1, ss.37-40, 2002 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 18 Sayı: 1
- Basım Tarihi: 2002
- Doi Numarası: 10.1023/a:1013917611796
- Dergi Adı: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.37-40
- Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet
Özet
Lipases have bio-imprinted with common substrate-interfaces and interesterification activities compared with amphiphile bio-imprinted counterparts. Bio-imprinting has yielded a 3.5- to 4.5-fold activity enhancement. Solvent-free medium was equally effective as hexane medium. Water addition erased the bio-imprinting effect. Bio-imprinting caused rate acceleration in the interesterification reaction and increased thermostability of the enzyme.