Improving the application of microbial lipase by bio-imprinting at substrate-interfaces


Yilmaz E.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, vol.18, no.1, pp.37-40, 2002 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 18 Issue: 1
  • Publication Date: 2002
  • Doi Number: 10.1023/a:1013917611796
  • Title of Journal : WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Page Numbers: pp.37-40

Abstract

Lipases have bio-imprinted with common substrate-interfaces and interesterification activities compared with amphiphile bio-imprinted counterparts. Bio-imprinting has yielded a 3.5- to 4.5-fold activity enhancement. Solvent-free medium was equally effective as hexane medium. Water addition erased the bio-imprinting effect. Bio-imprinting caused rate acceleration in the interesterification reaction and increased thermostability of the enzyme.