Biocompatible poly(galacturonic acid) micro/nanogels with controllable degradation via tunable chemical crosslinking

SUNER S. C., Ari B., SÜTEKİN S. D., ŞAHİNER N.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.201, ss.351-363, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 201
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.ijbiomac.2021.12.107
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.351-363
  • Anahtar Kelimeler: Polygalacturonic acid, Biodegradable microgels, Porous biocompatible materials, SHORT-CHAIN HYALURONATE, POLYGALACTURONIC ACID, DRUG-DELIVERY, PECTIN, HYDROGEL, MICROPARTICLES, FABRICATION, NANOGELS, SURVIVAL, POLYMER
  • Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet

Özet

Here, one-pot labor-less preparation of two different polygalacturonic acid (PGA) micro/nanogel formulations, PGA-1 and PGA-2, by respectively crosslinking the PGA chains with divinyl sulfone (DVS) and trimethylolpmpane triglycidyl ether (TMPGDE) were reported. Various crosslinker ratios, 2.5, 10, 50, and 100% were used for both crosslinkers to demonstrate the tunability of their degradation properties. The PGA micro/nanogels were found spherical-shaped porous particles in 0.5-5.0 mu m size range by SEM. The hydrolytic degradation and stability of PGA micro/nanogels in pH 1.0, 7.4, and 9.0 buffer solutions can be controlled by changing the degree of crosslinking. Accordingly, 32 +/- 8% and 36 +/- 2% weight losses were attained for PGA-1-10% and PGA-2-10% micro/nanogels at pH 1, respectively, and 46 +/- 6%, and 68 +/- 6% degradations were determined at pH 7.4 within 4 weeks. However, no degradation was observed for both PGA-based micro/nanogel formulations prepared at 25% and 100% crosslinker ratios at all pH conditions. All PGA-based micro/nanogels were totally degraded within 7-10 days at pH 9.0. In the presence of pectinase and amyloglucosidase enzymes, all formulations of PGA micro/nanogels showed more than 80% degradation within 12 h. Furthermore, both PGA formulations showed no significant cytotoxicity against L929 fibroblast cells with 90% and above cell viability up to 250 mg/mL concentrations.