Dichodontium pellucidum (Hedw.) Schimp. is a moss species belonging to the family Dicranaceae. It is distributed in various regions around the world, including Europe, Asia, North America, and Africa. In this study, antimicrobial, antibiofilm and antioxidant potentials four extracts (ethanol, methanol, acetone and ethyl acetate) of D. pellucidum were investigated. Disc diffusion, minimum inhibitory concentration, and minimum bactericidal concentration tests were performed to evaluate the antimicrobial activities. The effects of moss extracts on biofilm formation (antibiofilm) were evaluated based on crystal violet binding assay. The antioxidant activity of the extracts were measured by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a free radical scavenging method. The extracts of the moss have exhibited antimicrobial activity against all tested microorganisms with inhibition zones ranged from 8.0 to 16.0 mm for bacteria, 8.0 to 13.0 mm for the yeast culture. The highest antimicrobial and antibiofilm activity was achieved in ethyl acetate extract against the bacterium B. subtilis ATCC 6633. It was observed that the methanol solvent exhibited an effective antioxidant activity than other solvents. Obtained findings proved that the D. pellucidum frequently preferred in the investigation of natural antimicrobial and antioxidant agents and could be preferred for surveys of biofilm control due to their high potentials. This is the first report on the bioactivities of D. pellucidum. This approach may also allow new kind of research in medicinal usage or development of drug research.