Research Information System
PHYTOCHEMISTRY LETTERS, no.103503, pp.103503, 2025 (SCI-Expanded)
Ammi visnaga is a medicinal plant known by common names such as toothpick plant, toothpick herb, bisnaga, khella (Khalil, 2020). Visnagin is an important raw material in A. visnaga as it has a vasodilator effect that lowers blood pressure in rats (Lee et al., 2010). In the study, visnagin transformation was monitored during the bioreaction process in order to determine the visnagin level with Rhizobium rhizogenes inoculation. A. visnaga was propagated under tissue culture conditions with Murashige and Skoog (MS) medium containing 0.5mg.L-1+ 0.1mg.L-1 hormones. Internodes were used to transform friable callus with MS medium containing 2.0mg.L-1 2.4-D and were infected with R. rhizogenes. Aplikon my-control bioreactor was used to carry out the transformation conditions. Khellin levels were determined by spectrophotometric method under 390 mu (Karawya et al. 1971). In the study, no positive effect of bacterial inoculation on visnagin yield was observed.