Limited information is available on fatty acid and mineral composition of cluster bean (Cyamopsis tetragonoloba) genotype seeds grown in Mediterranean climates including Turkey. This study aimed to evaluate seed composition of thirty cluster bean genotypes according to Genotype Trait (GT) biplot analysis. Genotype fatty acid biplot captured 77.6% and genotype micro element biplot captured 65.3% of variation. The results of the present study indicated that oleic acid ranged from 20.07 to 33.62%, linoleic acid ranged from 31.36 to 40.72% in all genotypes. Concentrations of the six micronutrient elements were considerably different in genotypes. Fe and Mn are the most commonly found elements in cluster bean genotypes. Our results depicted that G13 was the most favorable genotype according to fatty acid profile, while G4 and G10 had the highest Fe and Cu contents, and G6 was the most stable genotype according to micronutrient profiles.