Akademik Veri Yönetim Sistemi
Mutation Research - Genetic Toxicology and Environmental Mutagenesis, cilt.902, 2025 (SCI-Expanded)
A growing amount of plastic waste is finding its way into natural ecosystems as a result of the widespread usage of plastics in modern society. These wastes degrade physically and biologically over time, transforming into microplastics (MPs) and nanoplastics (NPs). MPs and NPs emissions from the terrestrial environment then mix with rivers and eventually the seas, forming garbage. The cytotoxic and genotoxic effects of 50 nm polystyrene nanoplastics (PsNP) on human lymphocytes were assessed using the in vitro mitotic index (MI), micronucleus (MN), and comet assays. Both 24 and 48-h applications were performed for MI, and it was determined that 50 nm PsNP provided a statistically significant decrease in MI compared to the control at all concentrations and application times (except 0.001 and 0.1 μg/mL at 24 h). According to the MN test results, the MN frequency increased significantly at all concentrations when compared to the negative control. In the comet test, a statistically significant increase of comet tail length was observed at 0.001, 10 and 100 μg/mL concentration with 50 nm PsNP exposure. Tail moment also showed a statistically significant increase at the lowest concentration of 0.001 μg/mL and the highest concentration of 1, 10, 100 μg/mL compared to the negative control. All test results show that PsNP has both genotoxic and cytotoxic potential.