Biocompatible macro, micro and nano scale guar gum hydrogels and their protein absorption capacity

Suner S., ŞAHİNER N.

JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, vol.57, no.12, pp.810-818, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 12
  • Publication Date: 2020
  • Doi Number: 10.1080/10601325.2020.1787844
  • Journal Name: JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Biotechnology Research Abstracts, CAB Abstracts, Chimica, Communication Abstracts, Compendex, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.810-818
  • Keywords: Guar gum, hydrogel, microgel, nanogel, biocompatible, protein absorption, CROSS-LINKED GUAR, IN-VITRO, DRUG, HEMOCOMPATIBILITY
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

This work describes the preparation and characterization of different guar gum (GG) hydrogels at different sizes to be used for protein absorption. GG macrogels and micro/nanogels were synthesized through chemical crosslinkingviabulk polymerization and reverse micelle microemulsion techniques, respectively, and high yields, for example, 94 +/- 4% and 91 +/- 6% were obtained. GG micro/nanogels with spherical shape and smooth surface were separated in the range of 1-10 mu m and 300-900 nm by the filtration process. The blood compatibility assay revealed that GG micro/nanogels were blood compatible up to 2 mg/mL concentration with maximum 1.59 +/- 0.97% hemolysis ratio and minimum 84 +/- 2.6% blood clotting index. Furthermore, the cytotoxicity assay demonstrated that linear GG inhibited cell viability at >= 250 mu g/mL concentrations, whereas no significant difference was obtained up to 1000 mu g/mL concentration when compared to the control groups statistically (p > .05). It was further demonstrated that GG micro/nanogels retain great potential to absorb hemoglobin and lysozyme with 59 +/- 7 and 20 +/- 8 mg/g absorption capacity at pH 7.4. Furthermore, the absorption capacity of the negatively charged GG micro/nanogels could be augmented by adjusting the pH conditions down to the isoelectric point (IP) of the proteins.