In the study, the effects of different feeding programs on some blood biochemistry and tonic immobility durations in Ross 308 broilers were investigated. When aged 7 d, 60 chicks were divided into three groups to this end as AD, FR and NF in such a way that each group contained 20 birds. The tonic immobility durations, some parameters of blood biochemistry and the H:L ratios, by utilizing the heterophil and lymphocyte counts were determined. As a result of the analysis of variance carried out, it was observed that the H:L ratios of the birds in group NF were higher than those of the birds in groups AD and FR (p = 0.031). No statistically significant difference existed between the tonic immobility durations of the birds in groups AD and FR (p > 0.05) whereas it was observed that the tonic immobility durations of the birds in group NF were clearly higher than those of the groups AD and FR (p = 0.021). Starting from these findings, it was concluded that increasing the duration of feed restriction caused a serious level of stress on birds.