In this study, the effects of sythetic chemicals and medicinal herbs on the amount of bacteria in egg water and egg hatching of the angel fish (Pterophyllum scalare) were investigated. In this goal, methylene blue exposed to 0.5 ppm and potassium permanganate exposed to 0.2 and 0.4 ppm. Each rosemary, yarrow and sumac extracts exposed to 1.000, 5.000 and 10.000 ppm. Methylene blue (0.5 ppm), potassium permanganate (0.2 ppm), rosemary (1.000, 5.000, and 10.000 ppm) and yarrow (5.000 ppm) treatments were significantly decreased bacterial loads and increased egg hatching (P<0.05). With the increasing in the concentration of rosemary extract the bacterial loads decreased and egg hatching increased. All sumac treatments were significantly decreased total bacterial loads, but were negatively affected on egg hatching. The findings were shown that the best group was rosemary for egg hatching. Finally, this study showed that rosemary extract was more suitable to use instead of sythetic chemicals, and optimum rosemary extract dose was 1.000 ppm for the hatching of the angel fish eggs.