Copy For Citation
Tiryaki İ., Sarı U., Acar O., Kaya Ç., Çetin S.
Global Conference on Plant Science and Research, Valencia, Spain, 23 - 25 September 2019, pp.40
-
Publication Type:
Conference Paper / Full Text
-
City:
Valencia
-
Country:
Spain
-
Page Numbers:
pp.40
-
Çanakkale Onsekiz Mart University Affiliated:
Yes
Abstract
Drought is
one of the major abiotic stress
factors threatening agricultural production al
around .the world. Perennial legume plant alfalfa
(Medicago sativa) is the most important forage crop
of moderate climate reg10n. Along with deep root structure, mature alfalfa is generally considered as drought tolerant. However, alfalfa is especially sensitive
to drought at germination, seedling
and right after cutting. Various studies provided that polyethylene glycol (PEG) can successfully be used to provide drought stress
at controlled conditions. The mutant M3 seeds were screened in a half strength root growth MS media containing 35% PEG under in vitro conditions to create drought stress environment in a root growth medium. Tolerant genotypes were determined in the screening and were transplanted
to pots and then be grown in growth chamber. Candidate drought tolerant mutant genotypes were grazed at 5 cm cutting height when the first flowering buds visible and drought stress was
applied for 24 days. Some morphological, physiological (Protein, l\1DA, APX and SOD enzyme activities) and molecular aspects
(drought related gene expression levels) of drought
response parameters were determined and were compared
with control plants.