In vitro screening of mutant medicago sativa genotypes for drought stress tolerance at germination stage

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Tiryaki İ., Sarı U., Acar O., Kaya Ç., Çetin S.

Global Conference on Plant Science and Research, Valencia, Spain, 23 - 25 September 2019, pp.40

  • Publication Type: Conference Paper / Full Text
  • City: Valencia
  • Country: Spain
  • Page Numbers: pp.40
  • Çanakkale Onsekiz Mart University Affiliated: Yes



Drought is one of the major abiotic stress factors threatening agricultural production al around .the world. Perennial legume plant alfalfa (Medicago sativa) is the most important forage crop of moderate climate reg10n. Along with deep root structure, mature alfalfa is generally considered as drought tolerant. However, alfalfa is especially sensitive to drought at germination, seedling and right after cutting. Various studies provided that polyethylene glycol (PEG) can successfully be used to provide drought stress at controlled conditions. The mutant M3 seeds were screened in a half strength root growth MS media containing 35% PEG under in vitro conditions to create drought stress environment in a root growth medium. Tolerant genotypes were determined in the screening and were transplanted to pots and then be grown in growth chamber. Candidate drought tolerant mutant genotypes were grazed at 5 cm cutting height when the first flowering buds visible and drought stress was applied for 24 days. Some morphological, physiological (Protein, l\1DA, APX and SOD enzyme activities) and molecular aspects (drought related gene expression levels) of drought response parameters were determined and were compared with control plants.