Mixing of a shear thinning fluid was scaled-up by maintaining equal Reynolds number (Re), tip speed, and power per volume. A standard 45 degrees four-blade pitch blade turbine (PBT) and a modified version of the same impeller (DF-PBT) which provided simultaneous upward and downward flow were used. The impellers were located eccentrically, and the effects of scale-up were determined. The distribution of a tracer solution to evaluate mixing progress, power consumption, and flow fields were analyzed to compare the three different scale-up rules. The same endpoint of mixing was achieved in all cases of PBT with noticeable differences in flow profiles. Higher power consumption and less time were necessary to complete mixing with equal power per volume compared to that with equal Re and tip speed rules. For DF-PBT, only equal Re rule resulted in values similar to that obtained at the small scale. At a scale-up ratio of 2, the selection of scale-up rule should be based on the time and power requirements of the process. As the effects of scale-up on eccentrically located impellers do not differ significantly from those of concentrically located impellers, impellers which have complex dynamics such as DF-PBT require further studies to understand scale-up effects.