In present study, removal of methylene blue (MB) was investigated to the best removal efficiency using fish bones, which are biogenic apatite, natural origin and waste product. Removal efficiency of the adsorbent was investigated as a function of contact time and initial dye concentration. Highest removal capacity was found to be 13.56 mgg(-1). Langmuir and Freundlich models were also applied to experimental data to find the best adsorption isotherm. Experimental data showed that adsorption of methylene blue on the fish bones fitted well to Langmuir isotherm model, and also the value of R-L was found to be 0.765. R-L value of bone adsorbent between 0 and 1 (0 < R-L < 1) indicate favorable removal and adsorption of the dye. Results were showed that fish bones can be effectively used as an adsorbent for the removal of cationic dyes from aqueous environments.