Heat production from chickens and other resources inside the broiler house is a major problem for the broiler industry. Temperature and relative humidity (RH) as well as airflow and broiler physiology directly affect heat production in broiler houses. This study was carried out under laboratory conditions to determine heat production for male broilers. In the laboratory experiment, 108 broilers were used to determine the influence of different temperature-humidity combinations on the physiological response of 2.0 to 2.3 kg broilers during the 40 min test. In the laboratory experiment, three dry bulb (DB) temperatures (25 degrees C, 30 degrees C, and 35 degrees C) and three RHs (50%, 70%. and 90%) were used in a block design with four replications, 3 birds per test. DB and wet bulb (WB) temperatures of air entering and exiting the chamber were measured. Before and after each test, body temperatures were measured to control the stress level, and sensible heat production (SHP) and latent heat production (LHP) were calculated based on DB and WB temperatures.